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KMID : 1098419930010020184
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Korean Journal of Medicinal Crop Science
1993 Volume.1 No. 2 p.184 ~ p.190
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Callus Induction and Somatic Embryogenesis in Suspension Culture of Rehmannia glutinosa
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채영암/Chae, Young Am
박상언/Park, Sang Un
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Abstract
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This study was carried out to investigate the appropriate medium and constitutionof growth regulators for somatic embryogenesis for development of rapid mass propagation system via somatic embrygenesis in Rehmennia glutinosa. Embryogenic callus formation from leaf explant was more effective when 4mg / l BA with 0.5mg / l NAA than that of treated with only auxins or cytokinins. LS medium was suitable for embryogenic callus formation. LS medium with 4mg / l BA with 0.5mg / l NAA was effective for the maintenance and proliferation of embryogenic callus. In suspension culture, addition of 1mg / l BA to LS medium was proper for somatic embryogenesis. The highest rate of shoot developement form cotyledon stage embryo was obtained in 1/2 LS medium and plantlet survived by 75% after transplanted to the soil. after 4 weeks.
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